Genetic Profiling of the Full-Length tprK Gene in Patients with Primary and Secondary Syphilis.

ABSTRACT: TprK antigenic variation is acknowledged as an important strategy developed by Treponema pallidum to achieve immune evasion. Previous studies applied short-read sequencing to explore tprK gene sequence diversity in clinical samples; however, due to the limitations of short-read sequencing, it was difficult to determine the linkage between the seven V regions, and crucial information about full-length tprK variants was lost. Although two recent studies explored complete tprK gene profiles in natural human syphilis infection, there are still too few profiled full-length tprK variants among clinical T. pallidum isolates to fully understand the characteristics of TprK coding diversity. Here, Pacific Biosciences (PacBio) long-read sequencing was applied to examine th

SUBMITTER: Liu D 

PROVIDER: S-EPMC10269439 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature