Identification of Salmonella Pullorum Factors Affecting Immune Reaction in Macrophages from the Avian Host.

ABSTRACT: The host-specific Salmonella serovar S. Pullorum (SP) modulates the chicken immune response to a Th2-biased response associated with persistent infection. This is different from the Th1-biased immune response induced by the genetically close serovar, S. Enteritidis (SE). Based on core genome differences between SP and SE, we used three complementary bioinformatics approaches to identify SP genes, which may be important for stimulation of the immune response. Defined mutants were constructed in selected genes, and the infection potential and ability of mutants to stimulate cytokine production in avian derived HD11 macrophages were determined. Deletion of large genomic regions unique to SP did not change infection potential nor immune stimulation significantly. Mutants in genes

SUBMITTER: Fei X 

PROVIDER: S-EPMC10269470 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature