Characterization of Three Novel IMP Metallo-β-Lactamases, IMP-89, IMP-91, and IMP-96, and Diverse bla_IMP-Carrying Accessory Genetic Elements from Chinese Clinical Isolates.

ABSTRACT: Three novel imipenemase (IMP)-type metallo-β-lactamases (MBLs), referred to as IMP-89, IMP-91, and IMP-96, were detected in three clinical isolates from China. Antimicrobial susceptibility tests indicated these novel enzymes were resistant to most β-lactams, and IMP-96 with a Ser262Gly mutation had higher activity against meropenem than its point mutant. We then collected sequence data on all 91 available IMP variants for phylogenetic analysis. To further analyze the genetic environment of bla_IMP, an extensive comparison was applied to nine accessory genetic elements (AGEs), including six sequenced bla_IMP-carrying AGEs in this study and three others from GenBank. These nine AGEs were divided into three groups: three Inc_pJBCL41 plasmids, Tn6

SUBMITTER: Li X 

PROVIDER: S-EPMC10269577 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature