OmpC-Dependent Bile Tolerance Contributes to E. coli Colonization of the Mammalian Intestine.

ABSTRACT: Escherichia coli persistently colonizes the mammalian intestine by mechanisms that are not fully understood. Previously, we found when streptomycin-treated mice were fed E. coli MG1655, the intestine selected for envZ missense mutants that outcompeted the wild type. The better-colonizing envZ mutants had a higher level of OmpC and reduced OmpF. This suggested the EnvZ/OmpR two-component system and outer membrane proteins play a role in colonization. In this study, we show that wild-type E. coli MG1655 outcompetes an envZ-ompR knockout mutant. Moreover, ompA and ompC knockout mutants are outcompeted by the wild type, while an ompF knockout mutant colonizes better than the wild type. Outer membrane protein gels show the ompF mutant overproduce

SUBMITTER: Doranga S 

PROVIDER: S-EPMC10269588 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature