Development of a Serum-Free Medium To Aid Large-Scale Production of Mycoplasma-Based Therapies.

ABSTRACT: To assist in the advancement of the large-scale production of safe Mycoplasma vaccines and other Mycoplasma-based therapies, we developed a culture medium free of animal serum and other animal components for Mycoplasma pneumoniae growth. By establishing a workflow method to systematically test different compounds and concentrations, we provide optimized formulations capable of supporting serial passaging and robust growth reaching 60 to 70% of the biomass obtained in rich medium. Global transcriptomic and proteomic analysis showed minor physiological changes upon cell culture in the animal component-free medium, supporting its suitability for the production of M. pneumoniae-based therapies. The major contributors to growth performance were found to be glucose as a carbon sour

SUBMITTER: Burgos R 

PROVIDER: S-EPMC10269708 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature