Heterogeneity among Clinical Intestinal Escherichia coli Isolates upon Acquired Streptomycin Resistance.

ABSTRACT: Escherichia coli isolates from inflammatory bowel disease (IBD) patients are often multidrug resistant, including to streptomycin. Streptomycin resistance (Str^R) mutations can alter bacterial behavior, which may influence intestinal disease. We generated a spontaneous Str^R strain of the intestinal adherent-invasive E. coli (AIEC) strain NC101. Whole-genome sequencing revealed a single missense mutation in rpsL that commonly confers Str^R, rpsL-K43N. Str^R NC101 exhibited a striking loss of aggregation and significantly increased motility, behaviors that can impact host-microbe interactions. Behavioral changes were associated with reduced transcription of csgA, encoding the biofilm component curli, and increased transcription of

SUBMITTER: Lopez LR 

PROVIDER: S-EPMC10269711 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature