Enrichment Culture but Not Metagenomic Sequencing Identified a Highly Prevalent Phage Infecting <i>Lactiplantibacillus plantarum</i> in Human Feces.

ABSTRACT: Lactiplantibacillus plantarum (previously known as Lactobacillus plantarum) is increasingly used as a probiotic to treat human diseases, but its phages in the human gut remain unexplored. Here, we report its first gut phage, Gut-P1, which we systematically screened using metagenomic sequencing, virus-like particle (VLP) sequencing, and enrichment culture from 35 fecal samples. Gut-P1 is virulent, belongs to the Douglaswolinvirus genus, and is highly prevalent in the gut (~11% prevalence); it has a genome of 79,928 bp consisting of 125 protein coding genes and displaying low sequence similarities to public L. plantarum phages. Physiochemical characterization shows that it has a short latent period and adapts to broad ranges of temperatures and pHs. Furthermore, Gut-P1 strongly

SUBMITTER: Zhao X 

PROVIDER: S-EPMC10269749 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature