Culture-Independent Workflow for Nanopore MinION-Based Sequencing of Influenza A Virus.

ABSTRACT: Whole-genome sequencing (WGS) of influenza A virus (IAV) is crucial for identifying diverse subtypes and newly evolved variants and for selecting vaccine strains. In developing countries, where facilities are often inadequate, WGS is challenging to perform using conventional next-generation sequencers. In this study, we established a culture-independent, high-throughput native barcode amplicon sequencing workflow that can sequence all influenza subtypes directly from a clinical specimen. All segments of IAV in 19 clinical specimens, irrespective of their subtypes, were amplified simultaneously using a two-step reverse transcriptase PCR (RT-PCR) system. First, the library was prepared using the ligation sequencing kit, barcoded individually using the native barcodes, and sequenced on the Mi

SUBMITTER: Miah M 

PROVIDER: S-EPMC10269883 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature