Exosomal circ-AHCY promotes glioblastoma cell growth via Wnt/β-catenin signaling pathway.

ABSTRACT:

Background

Glioblastoma (GBM) is the most aggressive brain tumor. Reportedly, circular RNAs (circRNAs) participate in regulation of the development and progression of diverse cancers, including GBM.

Methods

Dysregulated circRNAs in GBM tissues were screened out from GEO database. The expression of candidate circRNAs in GBM cells was measured by qRT-PCR. Loss-of function assays, including colony formation assay, EdU assay, TUNEL assay, and flow cytometry analysis were conducted to determine the effects of circ-AHCY knockdown on GBM cell proliferation and apoptosis. Animal study was further used to prove the inhibitory effect of circ-AHCY silencing on GMB cell growth. Mechanistic experiments like luciferase reporter, RNA pull-down and RNA-binding protein immunoprecipitation (R

SUBMITTER: Li Y 

PROVIDER: S-EPMC10270256 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature