TATA-box-binding protein promotes hepatocellular carcinoma metastasis through epithelial-mesenchymal transition.

ABSTRACT:

Background

HCC characterizes malignant metastasis with high incidence and recurrence. Thus, it is pivotal to discover the mechanisms of HCC metastasis. TATA-box-binding protein (TBP), a general transcriptional factor (TF), couples with activators and chromatin remodelers to sustain the transcriptional activity of target genes. Here, we investigate the key role of TBP in HCC metastasis.

Methods

TBP expression was measured by PCR, western blot, and immunohistochemistry. RNA-sequencing was performed to identify downstream proteins. Functional assays of TBP and downstream targets were identified in HCC cell lines and xenograft models. Luciferase reporter and chromatin immunoprecipitation assays were used to demonstrate the mechanism mediated by TBP.

Results

HCC patients s

SUBMITTER: Cao J 

PROVIDER: S-EPMC10270559 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature