Molecular characterization and functionality of rumen-derived extracellular vesicles using a Caenorhabditis elegans animal model.

ABSTRACT: The rumen fluids contain a wide range of bacteria, protozoa, fungi, and viruses. The various ruminal microorganisms in the rumen provide nutrients by fermenting the forage they eat. During metabolic processes, microorganisms present in the rumen release diverse vesicles during the fermentation process. Therefore, in this study, we confirmed the function of rumen extracellular vesicles (EVs) and their interaction with the host. We confirmed the structure of the rumen EVs by transmission electron microscope (TEM) and the size of the particles using nanoparticle tracking analysis (NTA). Rumen EVs range in size from 100 nm to 400 nm and are composed of microvesicles, microparticles, and ectosomes. Using the Caenorhabditis elegans smart animal model, we verified the interaction between t

SUBMITTER: Choi H 

PROVIDER: S-EPMC10271931 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature