Transient depletion of macrophages alters local inflammatory response at the site of disc herniation in a transgenic mouse model.

ABSTRACT:

Objective

Macrophages are abundantly detected at sites of disc herniation, however, their function in the disease progression is unclear. We aim to investigate the functions of macrophages in acute disc herniation using a macrophage Fas-induced apoptosis (MaFIA) transgenic mouse strain.

Method

To transiently deplete macrophages, a dimerizer, AP20187, or vehicle solution was administered via intraperitoneal injection to MaFIA mice immediately, day 1 and 2 after annular puncture induced disc herniation. Local infiltrated tissues at disc hernia and DRGs at corresponding levels were harvested to analyze immune cells and neuroinflammation on postoperative day (POD) 6 by flow cytometry and/or immunostaining. Mouse spines were harvested to analyze structures of degenerated discs an

SUBMITTER: Xiao L 

PROVIDER: S-EPMC10272080 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature