SEC-seq: association of molecular signatures with antibody secretion in thousands of single human plasma cells.

ABSTRACT: The secreted products of cells drive many functions in vivo; however, methods to link this functional information to surface markers and transcriptomes have been lacking. By accumulating secretions close to secreting cells held within cavity-containing hydrogel nanovials, we demonstrate workflows to analyze the amount of IgG secreted from single human B cells and link this information to surface markers and transcriptomes from the same cells. Measurements using flow cytometry and imaging flow cytometry corroborate the association between IgG secretion and CD38/CD138. By using oligonucleotide-labeled antibodies we find that upregulation of pathways for protein localization to the endoplasmic reticulum and mitochondrial oxidative phosphorylation are most associated with high IgG secretion, a

SUBMITTER: Cheng RY 

PROVIDER: S-EPMC10272111 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature