Metabolic engineering of Halomonas campaniensis strain XH26 to remove competing pathways to enhance ectoine production.

ABSTRACT: Ectoine has gained considerable attention as a high-value chemical with significant application potential and market demand. This study aimed to increase ectoine yields by blocking the metabolic shunt pathway of L-aspartate-4-semialdehyde, the precursor substrate in ectoine synthesis. The homoserine dehydrogenase encoded by hom in H. campaniensis strain XH26 is responsible for the metabolic shunt of L-aspartate-4-semialdehyde to glycine. CRISPR/Cas9 technology was used to seamlessly knockout hom, blocking the metabolic shunt pathway to increase ectoine yields. The ectoine yield of XH26/Δhom was 351.13 mg (g CDW)^-1 after 48 h of incubation in 500 mL shake flasks using optimal medium with 1.5 mol L^-1 NaCl, which was significantly higher than the 239.18 mg (g CDW)^-1

SUBMITTER: Shu Z 

PROVIDER: S-EPMC10272175 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature