Characterizing the Macrophage Population in Patients With Idiopathic Subglottic Stenosis.

ABSTRACT:

Objectives

Idiopathic subglottic stenosis (iSGS) is characterized by progressive fibrosis and subglottic luminal narrowing. Currently, immune characterization has focused on T-cells; however, macrophages remain largely unexplored. The goals of this study are to characterize the transcriptome of iSGS macrophages and the fibrogenic nature of identifed biomarkers.

Study design

Bioinformatics and in vitro.

Methods

Human tracheal biopsies from iSGS scar (n = 4), and matched non-scar (n = 4) regions were analyzed using single-cell RNA-seq (scRNA-seq). Immunofluorescence (IF) was performed on rapidly processed autopsies (RPA) and iSGS tracheal resections (n = 4) to co-localize S100A8/9 and CD11b. Collagen gene/protein expression was assessed in iSGS fibroblasts (n = 4) treat

SUBMITTER: Ospino R 

PROVIDER: S-EPMC10272290 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature