Schlafen4⁺-MDSC in <i>Helicobacter</i>-induced gastric metaplasia reveals role for GTPases.

ABSTRACT:

Introduction

MDSCs express SCHLAFEN 4 (SLFN4) in Helicobacter-infected stomachs coincident with spasmolytic polypeptide-expressing metaplasia (SPEM), a precursor of gastric cancer. We aimed to characterize SLFN4⁺ cell identity and the role of Slfn4 in these cells.

Methods

Single-cell RNA sequencing was performed on immune cells sorted from PBMCs and stomachs prepared from uninfected and 6-month H. felis-infected mice. Knockdown of Slfn4 by siRNA or PDE5/6 inhibition by sildenafil were performed in vitro. Intracellular ATP/GTP levels and GTPase activity of immunoprecipitated Slfn4 complexes were measured using the GTPase-Glo assay kit. The intracellular level of ROS was quantified by the DCF-DA fluorescent staining, and apoptosis was determined

SUBMITTER: Ding L 

PROVIDER: S-EPMC10272601 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature