Time-resolved proteomic analyses of senescence highlight metabolic rewiring of mitochondria.

ABSTRACT: Mitochondrial dysfunction and cellular senescence are hallmarks of aging. However, the relationship between these two phenomena remains incompletely understood. In this study, we investigated the rewiring of mitochondria upon development of the senescent state in human IMR90 fibroblasts. Determining the bioenergetic activities and abundance of mitochondria, we demonstrate that senescent cells accumulate mitochondria with reduced OXPHOS activity, resulting in an overall increase of mitochondrial activities in senescent cells. Time-resolved proteomic analyses revealed extensive reprogramming of the mitochondrial proteome upon senescence development and allowed the identification of metabolic pathways that are rewired with different kinetics upon establishment of the senescent state. Among th

SUBMITTER: Kim JY 

PROVIDER: S-EPMC10272782 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature