A conserved uORF in the ilvBNC mRNA of Corynebacterium species regulates ilv operon expression.

ABSTRACT: Computational methods can be used to identify putative structured noncoding RNAs (ncRNAs) in bacteria, which can then be validated using various biochemical and genetic approaches. In a search for ncRNAs in Corynebacterium pseudotuberculosis, we observed a conserved region called the ilvB-II motif located upstream of the ilvB gene that is also present in other members of this genus. This gene codes for an enzyme involved in the production of branched-chain amino acids (BCAAs). The ilvB gene in some bacteria is regulated by members of a ppGpp-sensing riboswitch class, but previous and current data suggest that the ilvB-II motif regulates expression by a transcription attenuation mechanism involving protein translation from an upstream open reading frame (u

SUBMITTER: Narunsky A 

PROVIDER: S-EPMC10272879 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature