Quantitative plasma profiling by ¹H NMR-based metabolomics: impact of sample treatment.

ABSTRACT: Introduction: There is evidence that sample treatment of blood-based biosamples may affect integral signals in nuclear magnetic resonance-based metabolomics. The presence of macromolecules in plasma/serum samples makes investigating low-molecular-weight metabolites challenging. It is particularly relevant in the targeted approach, in which absolute concentrations of selected metabolites are often quantified based on the area of integral signals. Since there are a few treatments of plasma/serum samples for quantitative analysis without a universally accepted method, this topic remains of interest for future research. Methods: In this work, targeted metabolomic profiling of 43 metabolites was performed on pooled plasma to compare four methodologies consisting of Carr-Purcell-Me

SUBMITTER: Madrid-Gambin F 

PROVIDER: S-EPMC10273206 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature