Novel Roles for Diacylglycerol in Synaptic Vesicle Priming and Release Revealed by Complete Reconstitution of Core Protein Machinery.

ABSTRACT: Here we introduce the full functional reconstitution of genetically-validated core protein machinery (SNAREs, Munc13, Munc18, Synaptotagmin, Complexin) for synaptic vesicle priming and release in a geometry that enables detailed characterization of the fate of docked vesicles both before and after release is triggered with Ca ²⁺ . Using this novel setup, we discover new roles for diacylglycerol (DAG) in regulating vesicle priming and Ca ^2+- triggered release involving the SNARE assembly chaperone Munc13. We find that low concentrations of DAG profoundly accelerate the rate of Ca ²⁺ -dependent release, and high concentrations reduce clamping and permit extensive spontaneous release. As expected, DAG also increases the number of ready-release vesicles. Dynami

SUBMITTER: Sundaram RVK 

PROVIDER: S-EPMC10274626 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature