A ligation-independent sequencing method reveals tRNA-derived RNAs with blocked 3' termini.

ABSTRACT: Despite the numerous sequencing methods available, the vast diversity in size and chemical modifications of RNA molecules makes the capture of the full spectrum of cellular RNAs a difficult task. By combining quasi-random hexamer priming with a custom template switching strategy, we developed a method to construct sequencing libraries from RNA molecules of any length and with any type of 3' terminal modification, allowing the sequencing and analysis of virtually all RNA species. Ligation-independent detection of all types of RNA (LIDAR) is a simple, effective tool to comprehensively characterize changes in small non-coding RNAs and mRNAs simultaneously, with performance comparable to separate dedicated methods. With LIDAR, we comprehensively characterized the coding and non-coding transcri

SUBMITTER: Scacchetti A 

PROVIDER: S-EPMC10274639 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature