Interleukin-21 Drives a Hypermetabolic State and CD4⁺ T Cell-associated Pathogenicity in Chronic Intestinal Inflammation.

ABSTRACT:

Background & aims

Incapacitated regulatory T cells (Tregs) contribute to immune-mediated diseases. Inflammatory Tregs are evident during human inflammatory bowel disease (IBD); however, mechanisms driving the development of these cells and their function are not well understood. Therefore, we investigated the role of cellular metabolism in Tregs relevant to gut homeostasis.

Methods

Using human Tregs, we performed mitochondrial ultrastructural studies via electron microscopy and confocal imaging, biochemical and protein analyses using proximity ligation assay, immunoblotting, mass cytometry and fluorescence-activated cell sorting, metabolomics, gene expression analysis, and real-time metabolic profiling utilizing Seahorse XF analyzer. We utilized Crohn's disease single-cell R

SUBMITTER: Bamidele AO 

PROVIDER: S-EPMC10274654 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature