Scalable projected Light Sheet Microscopy for high-resolution imaging of living and cleared samples.

ABSTRACT: Light sheet fluorescence microscopy (LSFM) is a widely used imaging technique for living and large cleared samples. However, high-performance LSFM systems are often prohibitively expensive and not easily scalable for high-throughput applications. Here, we introduce a cost-effective, scalable, and versatile high-resolution imaging framework, called projected Light Sheet Microscopy (pLSM), which repurposes readily available off-the-shelf consumer-grade components and an over-the-network control architecture to achieve high-resolution imaging of living and cleared samples. We extensively characterize the pLSM framework and showcase its capabilities through high-resolution, multi-color imaging and quantitative analysis of mouse and post-mortem human brain samples cleared using various techniqu

SUBMITTER: Chen Y 

PROVIDER: S-EPMC10274708 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature