Calling Cards: a customizable platform to longitudinally record protein-DNA interactions over time in cells and tissues.

ABSTRACT: Calling Cards is a platform technology to record a cumulative history of transient protein-DNA interactions in the genome of genetically targeted cell types. The record of these interactions is recovered by next generation sequencing. Compared to other genomic assays, whose readout provides a snapshot at the time of harvest, Calling Cards enables correlation of historical molecular states to eventual outcomes or phenotypes. To achieve this, Calling Cards uses the piggyBac transposase to insert self-reporting transposon (SRT) "Calling Cards" into the genome, leaving permanent marks at interaction sites. Calling Cards can be deployed in a variety of in vitro and in vivo biological systems to study gene regulatory networks involved in development, aging, and disease. Out of the

SUBMITTER: Yen A 

PROVIDER: S-EPMC10274760 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature