Rapid and Multiplexed Nucleic Acid Detection using Programmable Aptamer-Based RNA Switches.

ABSTRACT: Rapid, simple, and low-cost diagnostic technologies are crucial tools for combatting infectious disease. Here, we describe a class of aptamer-based RNA switches called aptaswitches that recognize specific target nucleic acid molecules and respond by initiating folding of a reporter aptamer. Aptaswitches can detect virtually any sequence and provide a fast and intense fluorescent readout, generating signals in as little as 5 minutes and enabling detection by eye with minimal equipment. We demonstrate that aptaswitches can be used to regulate folding of six different fluorescent aptamer/fluorogen pairs, providing a general means of controlling aptamer activity and an array of different reporter colors for multiplexing. By coupling isothermal amplification reactions with aptaswitches, we reac

SUBMITTER: Yan Z 

PROVIDER: S-EPMC10275000 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature