A simple and efficient method for PCR amplifiable DNA extraction from ancient bones.

ABSTRACT: A simple and effective modified ethanol precipitation-based protocol is described for the preparation of DNA from ancient human bones. This method is fast and requires neither hazardous chemicals nor special devices. After the powdering and incubating of the bone samples Dextran Blue was added as a carrier for removing the PCR inhibitors with selective ethanol precipitation. This method could eliminate the time-consuming separate decalcification step, dialysis, application of centrifugation-driven microconcentrators and the second consecutive PCR amplification. The efficiency of this procedure was demonstrated on ten 500-1200-year-old human bones from four different Hungarian burial sites. A mitochondrial specific primer pair was used to obtain sequence information from the purified ancien

SUBMITTER: Kalmar T 

PROVIDER: S-EPMC102752 | biostudies-literature | 2000 Jun

REPOSITORIES: biostudies-literature