Identification of a humanized mouse model for functional testing of immune-mediated biomaterial foreign body response.

ABSTRACT: Biomedical devices comprise a major component of modern medicine, however immune-mediated fibrosis and rejection can limit their function over time. Here, we describe a humanized mouse model that recapitulates fibrosis following biomaterial implantation. Cellular and cytokine responses to multiple biomaterials were evaluated across different implant sites. Human innate immune macrophages were verified as essential to biomaterial rejection in this model and were capable of cross-talk with mouse fibroblasts for collagen matrix deposition. Cytokine and cytokine receptor array analysis confirmed core signaling in the fibrotic cascade. Foreign body giant cell formation, often unobserved in mice, was also prominent. Last, high-resolution microscopy coupled with multiplexed antibody capture digit

SUBMITTER: Doloff JC 

PROVIDER: S-EPMC10275594 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature