Ribozyme activity modulates the physical properties of RNA-peptide coacervates.

ABSTRACT: Condensed coacervate phases are now understood to be important features of modern cell biology, as well as valuable protocellular models in origin-of-life studies and synthetic biology. In each of these fields, the development of model systems with varied and tuneable material properties is of great importance for replicating properties of life. Here, we develop a ligase ribozyme system capable of concatenating short RNA fragments into long chains. Our results show that the formation of coacervate microdroplets with the ligase ribozyme and poly(L-lysine) enhances ribozyme rate and yield, which in turn increases the length of the anionic polymer component of the system and imparts specific physical properties to the droplets. Droplets containing active ribozyme sequences resist growth, do n

SUBMITTER: Le Vay KK 

PROVIDER: S-EPMC10275638 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature