Using nanopore sequencing to identify fungi from clinical samples with high phylogenetic resolution.

ABSTRACT: The study of microbiota has been revolutionized by the development of DNA metabarcoding. This sequence-based approach enables the direct detection of microorganisms without the need for culture and isolation, which significantly reduces analysis time and offers more comprehensive taxonomic profiles across broad phylogenetic lineages. While there has been an accumulating number of researches on bacteria, molecular phylogenetic analysis of fungi still remains challenging due to the lack of standardized tools and the incompleteness of reference databases limiting the accurate and precise identification of fungal taxa. Here, we present a DNA metabarcoding workflow for characterizing fungal microbiota with high taxonomic resolution. This method involves amplifying longer stretches of ribosomal

SUBMITTER: Ohta A 

PROVIDER: S-EPMC10275880 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature