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Resource-aware construct design in mammalian cells.

ABSTRACT: Resource competition can be the cause of unintended coupling between co-expressed genetic constructs. Here we report the quantification of the resource load imposed by different mammalian genetic components and identify construct designs with increased performance and reduced resource footprint. We use these to generate improved synthetic circuits and optimise the co-expression of transfected cassettes, shedding light on how this can be useful for bioproduction and biotherapeutic applications. This work provides the scientific community with a framework to consider resource demand when designing mammalian constructs to achieve robust and optimised gene expression.

SUBMITTER: Di Blasi R

PROVIDER: S-EPMC10275982 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature

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Resource-aware construct design in mammalian cells.

Di Blasi Roberto R Pisani Mara M Tedeschi Fabiana F Marbiah Masue M MM Polizzi Karen K Furini Simone S Siciliano Velia V Ceroni Francesca F

Nature communications 20230616 1

Resource competition can be the cause of unintended coupling between co-expressed genetic constructs. Here we report the quantification of the resource load imposed by different mammalian genetic components and identify construct designs with increased performance and reduced resource footprint. We use these to generate improved synthetic circuits and optimise the co-expression of transfected cassettes, shedding light on how this can be useful for bioproduction and biotherapeutic applications. T ...[more]

PMID: 37328476

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