Ratiometric measurement of MAM Ca²⁺ dynamics using a modified CalfluxVTN.

ABSTRACT: Mitochondria-associated ER membrane (MAM) is a structure where these calcium-regulating organelles form close physical contact sites for efficient Ca²⁺ crosstalk. Despite the central importance of MAM Ca²⁺ dynamics in diverse biological processes, directly and specifically measuring Ca²⁺ concentrations inside MAM is technically challenging. Here, we develop MAM-Calflux, a MAM-specific BRET-based Ca²⁺ indicator. The successful application of the bimolecular fluorescence complementation (BiFC) concept highlights Ca²⁺-responsive BRET signals in MAM. The BiFC strategy imparts dual functionality as a Ca²⁺ indicator and quantitative structural marker specific for MAM. As a ratiometric Ca²⁺ indicator, MAM-Calflux estimat

SUBMITTER: Cho E 

PROVIDER: S-EPMC10276021 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature