Dataset Information

Chaperonin CCT controls extracellular vesicle production and cell metabolism through kinesin dynamics.

ABSTRACT: Cell proteostasis includes gene transcription, protein translation, folding of de novo proteins, post-translational modifications, secretion, degradation and recycling. By profiling the proteome of extracellular vesicles (EVs) from T cells, we have found the chaperonin complex CCT, involved in the correct folding of particular proteins. By limiting CCT cell-content by siRNA, cells undergo altered lipid composition and metabolic rewiring towards a lipid-dependent metabolism, with increased activity of peroxisomes and mitochondria. This is due to dysregulation of the dynamics of interorganelle contacts between lipid droplets, mitochondria, peroxisomes and the endolysosomal system. This process accelerates the biogenesis of multivesicular bodies leading to higher EV production through the dynamic regulation of microtubule-based kinesin motors. These findings connect proteostasis with lipid metabolism through an unexpected role of CCT.

SUBMITTER: Rojas-Gomez A

PROVIDER: S-EPMC10276179 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature

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Chaperonin CCT controls extracellular vesicle production and cell metabolism through kinesin dynamics.

Rojas-Gómez Amelia A Dosil Sara G SG Chichón Francisco J FJ Fernández-Gallego Nieves N Ferrarini Alessia A Calvo Enrique E Calzada-Fraile Diego D Requena Silvia S Otón Joaquin J Serrano Alvaro A Tarifa Rocio R Arroyo Montserrat M Sorrentino Andrea A Pereiro Eva E Vázquez Jesus J Valpuesta José M JM Sánchez-Madrid Francisco F Martín-Cófreces Noa B NB

Journal of extracellular vesicles 20230601 6

Cell proteostasis includes gene transcription, protein translation, folding of de novo proteins, post-translational modifications, secretion, degradation and recycling. By profiling the proteome of extracellular vesicles (EVs) from T cells, we have found the chaperonin complex CCT, involved in the correct folding of particular proteins. By limiting CCT cell-content by siRNA, cells undergo altered lipid composition and metabolic rewiring towards a lipid-dependent metabolism, with increased activi ...[more]

PMID: 37328936

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Project description:Please provide an overall description of your study, think something similar in scope to the manuscript abstractCell proteostasiCell proteostasis includes gene transcription, protein translation, folding of de novo proteins, post-translational modification, secretion, degradation and recycling. By profiling the proteome of extracellular vesicles (EVs) from T cells, we have found the chaperonin complex CCT, involved in the correct folding of particular proteins. By limiting CCT cell-content by siRNA, cells undergo altered lipid composition and metabolic rewiring towards a lipid-dependent metabolism, with increased activity of peroxisomes and mitochondria. This is due to dysregulation of the dynamics of interorganellar contacts between lipid droplets, mitochondria, peroxisomes and the endolysosomal system. This process accelerates the biogenesis of multivesicular bodies leading to higher EV production through the dynamic regulation of microtubule-based kinesin motors. These findings connect proteostasis with lipid metabolism through an unexpected role of CCT.s includes gene transcription, protein translation, folding of de novo proteins, post-translational modification, secretion, degradation and recycling. By profiling the proteome of extracellular vesicles (EVs) from T cells, we have found the chaperonin complex CCT, involved in the correct folding of particular proteins. By limiting CCT cell-content by siRNA, cells undergo altered lipid composition and metabolic rewiring towards a lipid-dependent metabolism, with increased activity of peroxisomes and mitochondria. This is due to dysregulation of the dynamics of interorganellar contacts between lipid droplets, mitochondria, peroxisomes and the endolysosomal system. This process accelerates the biogenesis of multivesicular bodies leading to higher EV production through the dynamic regulation of microtubule-based kinesin motors. These findings connect proteostasis with lipid metabolism through an unexpected role of CCT.

Dataset Information

Chaperonin CCT controls extracellular vesicle production and cell metabolism through kinesin dynamics.

Publications

Chaperonin CCT controls extracellular vesicle production and cell metabolism through kinesin dynamics.

Similar Datasets

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