Orthogonal approaches required to measure proteasome composition and activity in mammalian brain tissue.

ABSTRACT: Proteasomes are large macromolecular complexes with multiple distinct catalytic activities that are each vital to human brain health and disease. Despite their importance, standardized approaches to investigate proteasomes have not been universally adapted. Here, we describe pitfalls and define straightforward orthogonal biochemical approaches essential to measure and understand changes in proteasome composition and activity in the mammalian central nervous system. Through our experimentation in the mammalian brain, we determined an abundance of catalytically active proteasomes exist with and without a 19S cap(s), the regulatory particle essential for ubiquitin-dependent degradation. Moreover, we learned that in-cell measurements using activity-based probes (ABPs) are more sensitive in det

SUBMITTER: Turker F 

PROVIDER: S-EPMC10276296 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature