Reduced folate carrier 1 is present in retinal microvessels and crucial for the inner blood retinal barrier integrity.

ABSTRACT:

Background

Reduced folate carrier 1 (RFC1; SLC19a1) is the main responsible transporter for the B9 family of vitamins named folates, which are essential for normal tissue growth and development. While folate deficiency resulted in retinal vasculopathy, the expression and the role of RFC1 in blood-retinal barrier (BRB) are not well known.

Methods

We used whole mount retinas and trypsin digested microvessel samples of adult mice. To knockdown RFC1, we delivered RFC1-targeted short interfering RNA (RFC1-siRNA) intravitreally; while, to upregulate RFC1 we delivered lentiviral vector overexpressing RFC1. Retinal ischemia was induced 1-h by applying FeCl₃ to central retinal artery. We used RT-qPCR and Western blotting to determine RFC1. Endothelium (CD31), pericytes (PD

SUBMITTER: Gurler G 

PROVIDER: S-EPMC10276430 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature