Oral microbial communities in 5-year-old children with versus without dental caries.

ABSTRACT:

Background

Caries in young children has received more and more attention. The study of the oral microbiota may help to understand the polymicrobial etiology of dental caries.

Objectives

To investigate the diversity and structure of microbial communities in saliva samples from 5-year-old children with versus without dental caries.

Methods

A total of 36 saliva samples were collected from 18 children with high caries (HB group) and from 18 children without caries (NB group). Then, 16S rDNA was amplified from bacterial samples using polymerase chain reaction, and high-throughput sequencing was performed using Illumina Novaseq platforms.

Results

Sequences were clustered into operational taxonomic units (OTUs), which were distributed among 16 phyla, 26 classes, 56 or

SUBMITTER: Yang Z 

PROVIDER: S-EPMC10276436 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature