Released dsDNA-triggered inflammasomes serve as intestinal radioprotective targets.

ABSTRACT:

Objectives

Intestinal mucositis is the major side effect during abdominal or pelvic radiotherapy, but the underlying immunogen remains to be further characterised and few radioprotective agents are available. This study investigated the role of dsDNA-triggered inflammasomes in intestinal mucositis during radiotherapy.

Methods

Pro-inflammatory cytokines were detected by ELISA. Radiation-induced intestinal injury in mice was analyzed by means of survival curves, body weight, HE staining of intestines, and intestinal barrier integrity. Western blot, immunofluorescence staining, co-immunoprecipitation assay and flow cytometry were used to investigate the regulatory role of dsDNA on inflammasomes.

Results

Here, we show that a high level of IL-1β and IL-18 is associated wit

SUBMITTER: Chen L 

PROVIDER: S-EPMC10276537 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature