Expression, localization, and function of P4HB in the spermatogenesis of Chinese mitten crab (Eriocheir sinensis).

ABSTRACT:

Background

The sperm of Chinese mitten crab (Eriocheir sinensis) have special noncondensed nuclei. The formation and stability of the special nuclei are closely related to the correct folding of proteins during spermatogenesis. P4HB plays a key role in protein folding, but its expression and role in the spermatogenesis of E. sinensis are unclear.

Objective

To investigate the expression and distribution characteristics of P4HB in the spermatogenesis of E. sinensis as well as its possible role.

Methods

The testis tissues of adult and juvenile E. sinensis were used as materials. We utilized a variety of techniques, including homology modeling, phylogenetic analysis, RT-qPCR, western blotting, and immunofluorescence staining to predict the prot

SUBMITTER: Tang Y 

PROVIDER: S-EPMC10276555 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature