The NAD salvage pathway in mesenchymal cells is indispensable for skeletal development in mice.

ABSTRACT: NAD is an essential co-factor for cellular energy metabolism and multiple other processes. Systemic NAD⁺ deficiency has been implicated in skeletal deformities during development in both humans and mice. NAD levels are maintained by multiple synthetic pathways but which ones are important in bone forming cells is unknown. Here, we generate mice with deletion of Nicotinamide Phosphoribosyltransferase (Nampt), a critical enzyme in the NAD salvage pathway, in all mesenchymal lineage cells of the limbs. At birth, Nampt^ΔPrx1 exhibit dramatic limb shortening due to death of growth plate chondrocytes. Administration of the NAD precursor nicotinamide riboside during pregnancy prevents the majority of in utero defects. Depletion of NAD post-birth also promotes chondrocyte deat

SUBMITTER: Warren A 

PROVIDER: S-EPMC10276814 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature