Amplicon sequencing allows differential quantification of closely related parasite species: an example from rodent Coccidia (Eimeria).

ABSTRACT:

Background

Quantifying infection intensity is a common goal in parasitological studies. We have previously shown that the amount of parasite DNA in faecal samples can be a biologically meaningful measure of infection intensity, even if it does not agree well with complementary counts of transmission stages (oocysts in the case of Coccidia). Parasite DNA can be quantified at relatively high throughput using quantitative polymerase chain reaction (qPCR), but amplification needs a high specificity and does not simultaneously distinguish between parasite species. Counting of amplified sequence variants (ASVs) from high-throughput marker gene sequencing using a relatively universal primer pair has the potential to distinguish between closely related co-infecting taxa and to uncover the

SUBMITTER: Ferreira SCM 

PROVIDER: S-EPMC10276917 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature