V-ATPase-dependent ectodermal voltage and pH regionalization are required for craniofacial morphogenesis.

ABSTRACT: Using voltage and pH reporter dyes, we have discovered a never-before-seen regionalization of the Xenopus ectoderm, with cell subpopulations delimited by different membrane voltage and pH. We distinguished three courses of bioelectrical activity. Course I is a wave of hyperpolarization that travels across the gastrula. Course II comprises the appearance of patterns that match shape changes and gene expression domains of the developing face; hyperpolarization marks folding epithelium and both hyperpolarized and depolarized regions overlap domains of head patterning genes. In Course III, localized regions of hyperpolarization form at various positions, expand, and disappear. Inhibiting H(+) -transport by the H(+) -V-ATPase causes abnormalities in: (1) the morphology of craniofacial structure

SUBMITTER: Vandenberg LN 

PROVIDER: S-EPMC10277013 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature