Evaluation of silver bio-functionality in a multicellular in vitro model: towards reduced animal usage in implant-associated infection research.

ABSTRACT:

Background

Despite the extensive use of silver ions or nanoparticles in research related to preventing implant-associated infections (IAI), their use in clinical practice has been debated. This is because the strong antibacterial properties of silver are counterbalanced by adverse effects on host cells. One of the reasons for this may be the lack of comprehensive in vitro models that are capable of analyzing host-bacteria and host-host interactions.

Methods and results

In this study, we tested silver efficacy through multicellular in vitro models involving macrophages (immune system), mesenchymal stem cells (MSCs, bone cells), and S. aureus (pathogen). Our model showed to be capable of identifying each element of culture as well as tracking the intracell

SUBMITTER: Cecotto L 

PROVIDER: S-EPMC10277478 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature