Effect of gut microbiome regulated Taohong Siwu Decoction metabolism on glioma cell phenotype.

ABSTRACT:

Introduction

To establish a new model for exploring the mechanism of the gut microbiome and drug metabolism, we explored whether Taohong Siwu Decoction acts after metabolism by intestinal flora under the premise of clarifying the interaction between intestinal flora and drug metabolism.

Methods

Taohong Siwu Decoction (TSD) was fed to germ-free mice and conventional mice, respectively. The serum from both groups of mice was removed and co-cultured with glioma cells in vitro. The co-cultured glioma cells were compared separately for changes at the RNA level using RNA-seq technology. The genes of interest in the comparison results were selected for validation.

Results

The differences in the phenotypic alterations of glioma cells between serum from TSD-fed germ-free mice

SUBMITTER: Feng S 

PROVIDER: S-EPMC10277651 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature