How Is Substrate Halogenation Triggered by the Vanadium Haloperoxidase from Curvularia inaequalis?

ABSTRACT: Vanadium haloperoxidases (VHPOs) are unique enzymes in biology that catalyze a challenging halogen transfer reaction and convert a strong aromatic C-H bond into C-X (X = Cl, Br, I) with the use of a vanadium cofactor and H₂O₂. The VHPO catalytic cycle starts with the conversion of hydrogen peroxide and halide (X = Cl, Br, I) into hypohalide on the vanadate cofactor, and the hypohalide subsequently reacts with a substrate. However, it is unclear whether the hypohalide is released from the enzyme or otherwise trapped within the enzyme structure for the halogenation of organic substrates. A substrate-binding pocket has never been identified for the VHPO enzyme, which questions the role of the protein in the overall reaction mechanism. Probing its role in the halogenation

SUBMITTER: Gerard EF 

PROVIDER: S-EPMC10278073 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature