Building Large DNA Bundles via Controlled Hierarchical Assembly of DNA Tubes.

ABSTRACT: Structural DNA nanotechnology is capable of fabricating designer nanoscale artificial architectures. Developing simple and yet versatile assembly methods to construct large DNA structures of defined spatial features and dynamic capabilities has remained challenging. Herein, we designed a molecular assembly system where DNA tiles can assemble into tubes and then into large one-dimensional DNA bundles following a hierarchical pathway. A cohesive link was incorporated into the tile to induce intertube binding for the formation of DNA bundles. DNA bundles with length of dozens of micrometers and width of hundreds of nanometers were produced, whose assembly was revealed to be collectively determined by cationic strength and linker designs (binding strength, spacer length, linker position, etc.)

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC10278166 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature