Design and Selection of Heterodimerizing Helical Hairpins for Synthetic Biology.

ABSTRACT: Synthetic biology applications would benefit from protein modules of reduced complexity that function orthogonally to cellular components. As many subcellular processes depend on peptide-protein or protein-protein interactions, de novo designed polypeptides that can bring together other proteins controllably are particularly useful. Thanks to established sequence-to-structure relationships, helical bundles provide good starting points for such designs. Typically, however, such designs are tested in vitro and function in cells is not guaranteed. Here, we describe the design, characterization, and application of de novo helical hairpins that heterodimerize to form 4-helix bundles in cells. Starting from a rationally designed homodimer, we construct a library of helical h

SUBMITTER: Smith AJ 

PROVIDER: S-EPMC10278171 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature