Recapitulating the Lateral Organization of Membrane Receptors at the Nanoscale.

ABSTRACT: Many cell membrane functions emerge from the lateral presentation of membrane receptors. The link between the nanoscale organization of the receptors and ligand binding remains, however, mostly unclear. In this work, we applied surface molecular imprinting and utilized the phase behavior of lipid bilayers to create platforms that recapitulate the lateral organization of membrane receptors at the nanoscale. We used liposomes decorated with amphiphilic boronic acids that commonly serve as synthetic saccharide receptors and generated three lateral modes of receptor presentation─random distribution, nanoclustering, and receptor crowding─and studied their interaction with saccharides. In comparison to liposomes with randomly dispersed receptors, surface-imprinted liposomes resulted in more than

SUBMITTER: Tabaei SR 

PROVIDER: S-EPMC10278181 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature