Evaluation of Nanopore Sensor Design Using Electrical and Optical Analyses.

ABSTRACT: Nanopores are currently utilized as powerful tools for single-molecule protein sensing. The reporting signal typically requires protein analytes to enter the nanopore interior, yet a class of these sensors has emerged that allows targeted detection free in solution. This tactic eliminates the spatial limitation of nanopore confinement. However, probing proteins outside the nanopore implies numerous challenges associated with transducing the physical interactions in the aqueous phase into a reliable electrical signature. Hence, it necessitates extensive engineering and tedious optimization routes. These obstacles have prevented the widespread adoption of these sensors. Here, we provide an experimental strategy by developing and validating single-polypeptide-chain nanopores amenable to singl

SUBMITTER: Mayse LA 

PROVIDER: S-EPMC10278182 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature