Interlaboratory Reproducibility in Growth and Reporter Expression in the Cyanobacterium <i>Synechocystis</i> sp. PCC 6803.

ABSTRACT: In recent years, a plethora of new synthetic biology tools for use in cyanobacteria have been published; however, their reported characterizations often cannot be reproduced, greatly limiting the comparability of results and hindering their applicability. In this interlaboratory study, the reproducibility of a standard microbiological experiment for the cyanobacterial model organism Synechocystis sp. PCC 6803 was assessed. Participants from eight different laboratories quantified the fluorescence intensity of mVENUS as a proxy for the transcription activity of the three promoters P_J23100, P_rhaBAD, and P_petE over time. In addition, growth rates were measured to compare growth conditions between laboratories. By establishing strict

SUBMITTER: Mager M 

PROVIDER: S-EPMC10278186 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature