Optimized Signal Peptide for Secretory Expression of Human Recombinant Somatropin in E. coli.

ABSTRACT: Purpose: The human somatropin is a single-chain polypeptide with a pivotal role in various biological processes. Although Escherichia coli is considered as a preferred host for the production of human somatropin, the high expression of this protein in E. coli results in the accumulation of protein as inclusion bodies. Periplasmic expression using signal peptides could be used to overcome the formation of inclusion bodies; still, the efficiency of each of the signal peptides in periplasmic transportation is varied and often is protein specific. The present study aimed to use in silico analysis to identify an appropriate signal peptide for the periplasmic expression of human somatropin in E. coli. Methods: A library containing 90 prokar

SUBMITTER: Ahmadi Z 

PROVIDER: S-EPMC10278209 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature