Single-cell transcriptome profiling of sepsis identifies HLA-DR^lowS100A^high monocytes with immunosuppressive function.

ABSTRACT:

Background

Sustained yet intractable immunosuppression is commonly observed in septic patients, resulting in aggravated clinical outcomes. However, due to the substantial heterogeneity within septic patients, precise indicators in deciphering clinical trajectories and immunological alterations for septic patients remain largely lacking.

Methods

We adopted cross-species, single-cell RNA sequencing (scRNA-seq) analysis based on two published datasets containing circulating immune cell profile of septic patients as well as immune cell atlas of murine model of sepsis. Flow cytometry, laser scanning confocal microscopy (LSCM) imaging and Western blotting were applied to identify the presence of S100A9⁺ monocytes at protein level. To interrogate the immunosuppressive fu

SUBMITTER: Yao RQ 

PROVIDER: S-EPMC10278311 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature